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Image Search Results
Journal: Molecular Cancer Therapeutics
Article Title: Antitumor Activity of a Recombinant Soluble Ectodomain of Mutant Human Fibroblast Growth Factor Receptor-2 IIIc
doi: 10.1158/1535-7163.mct-11-0163
Figure Lengend Snippet: Figure 3. Effects of sFGFR2 on the proliferation and apoptosis of DU145 and MCF-7 cells. A, inhibition of the proliferation of MCF-7 and DU145 cells by sFGFR2 was measured using an MTT assay. When 20 ng/mL FGF-2 was added, 40 ng/mL sFGFR2 showed the highest inhibition of cell proliferation. The average value of 5 wells was calculated, and the experiment was repeated 3 times. B, an ELISA was used to determine the FGF-2 concentration in the culture media of DU145 and MCF-7 cells. C, effects of sFGFR2 (5 mg/mL) on the cell cycle were examined by FACS. Treatment with either msFGFR2 or wsFGFR2 resulted in G1 arrest and the inhibition of cell proliferation in DU145, MCF-7, and NIH3T3 cells. D, effects of sFGFR2 (5 mg/mL) on the apoptosis of DU145, MCF-7, and NIH3T3 cells were measured by Annexin V/PI double staining. MsFGFR2 was found to significantly induce apoptosis.
Article Snippet:
Techniques: Inhibition, MTT Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Double Staining